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CRISPR Design
(Page View: 5548)
Search for target sites by gene ID
PAM Sequence
NGG (SpCas9 from Streptococcus pyogenes: 5'-NGG-3')
NRG (SpCas9 from Streptococcus pyogenes: 5'-NRG-3'(R = A, G))
NNAGAAW (StCas9 from Streptococcus thermophilus: 5'-NNAGAAW-3'(W = A, T))
NNNNGMTT (NmCas9 from Neisseria meningitidis: 5'-NNNNGMTT-3'(M = A, C))
NNGRRT (SaCas9 from Staphylococcus aureus: 5'-NNGRRT-3'(R = A, G))
TTTN (AsCpf1 from Acidaminococcus or LbCpf1 from Lachnospiraceae: 5'-TTTN-3')
TTN (FnCpf1 from Francisella: 5'-TTN-3')
YCN (from Pyrococcus furiosus: 5'-YCN-3'(Y = T, C))
CCW (from Clostridium difficile: 5'-CCW-3'(W = A, T))
YYC (from Bacillus halodurans: 5'-YYC-3'(Y = T, C))
AWG (from Escherichia coli: 5'-AWG-3'(W = A, T))
CC (from Pseudomonas aeruginosa: 5'-CC-3')
MMA (Pyrococcus furiosus: 5'-MMA-3'(M = A, C))
Target Genome
Citrus australasica v1.0
Citrus clementine v1.0
Fortunella hindsii v1.0
Citrus hongheensis v1.0
Atalantia buxfoliata v1.0
Atalantia buxfoliata v2.0
Citrus grandis (L.) Osbeck.cv.'Wanbaiyou' v1.0
Murraya paniculata v1.0
Citrus reticulata v1.0
Citrus reticulata v2.0
Citrus linwuensis v1.0
Citrus mangshanensis v1.0
Citrus reticulata Blanco cv. Ponkan v1.0
Citrus medica v1.0
Citrus medica v2.0
Citrus sinensis v1.0
Citrus sinensis v2.0
Citrus sinensis v3.0
Citrus grandis (L.) Osbeck.cv.'Majiayou' v1.0
Citrus ichangensis v1.0
Citrus ichangensis v2.0
Citrus grandis (L.) Osbeck.cv.'Zipiyou' v1.0
Poncirus trifoliata v1.0
Max Mismatch
0
1
2
3
4
5
Guide Sequence Length
15
16
17
18
19
20
21
22
Gene ID
Example
Search for target sites by region
PAM Sequence
NGG (SpCas9 from Streptococcus pyogenes: 5'-NGG-3')
NRG (SpCas9 from Streptococcus pyogenes: 5'-NRG-3'(R = A, G))
NNAGAAW (StCas9 from Streptococcus thermophilus: 5'-NNAGAAW-3'(W = A, T))
NNNNGMTT (NmCas9 from Neisseria meningitidis: 5'-NNNNGMTT-3'(M = A, C))
NNGRRT (SaCas9 from Staphylococcus aureus: 5'-NNGRRT-3'(R = A, G))
TTTN (AsCpf1 from Acidaminococcus or LbCpf1 from Lachnospiraceae: 5'-TTTN-3')
TTN (FnCpf1 from Francisella: 5'-TTN-3')
YCN (from Pyrococcus furiosus: 5'-YCN-3'(Y = T, C))
CCW (from Clostridium difficile: 5'-CCW-3'(W = A, T))
YYC (from Bacillus halodurans: 5'-YYC-3'(Y = T, C))
AWG (from Escherichia coli: 5'-AWG-3'(W = A, T))
CC (from Pseudomonas aeruginosa: 5'-CC-3')
MMA (Pyrococcus furiosus: 5'-MMA-3'(M = A, C))
Target Genome
Citrus australasica v1.0
Citrus clementine v1.0
Fortunella hindsii v1.0
Citrus hongheensis v1.0
Atalantia buxfoliata v1.0
Atalantia buxfoliata v2.0
Citrus grandis (L.) Osbeck.cv.'Wanbaiyou' v1.0
Murraya paniculata v1.0
Citrus reticulata v1.0
Citrus reticulata v2.0
Citrus linwuensis v1.0
Citrus mangshanensis v1.0
Citrus reticulata Blanco cv. Ponkan v1.0
Citrus medica v1.0
Citrus medica v2.0
Citrus sinensis v1.0
Citrus sinensis v2.0
Citrus sinensis v3.0
Citrus grandis (L.) Osbeck.cv.'Majiayou' v1.0
Citrus ichangensis v1.0
Citrus ichangensis v2.0
Citrus grandis (L.) Osbeck.cv.'Zipiyou' v1.0
Poncirus trifoliata v1.0
Max Mismatch
0
1
2
3
4
5
Guide Sequence Length
15
16
17
18
19
20
21
22
Position
Example
